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Immunofluorescence and flow cytometry analysis of BCSFV-E2 cell passages.

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posted on 2014-09-08, 03:08 authored by Rong-Hong Hua, Hong Huo, Ye-Nan Li, Yao Xue, Xiao-Lei Wang, Li-Ping Guo, Bin Zhou, Yong Song, Zhi-Gao Bu

The tenth (F10), twentieth (F20), and thirtieth (F30) generations of BCSFV cells were analyzed by indirect immunofluorescence and flow cytometry. Both assays show a high percentage of cells expressing CSFV E2.

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Keywords

mE 2 protein CSFV E 2 protein Fever Virus E 2 Glycoprotein control group mE 2 neutralizing antibody titer CSFV subunit vaccine neutralizing antibody titers vaccination Stable Transgenic Mammalian Cell Line Classical swine fever virus tcid challenge envelope E 2 glycoprotein

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