Fig_9.tif (3.96 MB)

Immunity to powdery mildews is impaired in crk mutants.

figure

posted on 2015-07-21, 04:23 authored by Gildas Bourdais, Paweł Burdiak, Adrien Gauthier, Lisette Nitsch, Jarkko Salojärvi, Channabasavangowda Rayapuram, Niina Idänheimo, Kerri Hunter, Sachie Kimura, Ebe Merilo, Aleksia Vaattovaara, Krystyna Oracz, David Kaufholdt, Andres Pallon, Damar Tri Anggoro, Dawid Glów, Jennifer Lowe, Ji Zhou, Omid Mohammadi, Tuomas Puukko, Andreas Albert, Hans Lang, Dieter Ernst, Hannes Kollist, Mikael Brosché, Jörg Durner, Jan Willem Borst, David B. Collinge, Stanisław Karpiński, Michael F. Lyngkjær, Silke Robatzek, Michael Wrzaczek, Jaakko Kangasjärvi

(A) Relative amount of plant foliar mildew coverage (in percent) caused by the virulent biotrophic powdery mildew Golovinomyces orontii (Go) on crks compared to Col-0 wild type and Go-super-susceptible eds1. Results are mean ± SE (n = 15). The experiment was conducted three times and the amount of disease was normalised between experiments by setting the infection cover of Col-0 to one. Asterisks indicate differences between crks and Col-0, statistical significance *P<0.05, **P<0.01 and ***P<0.001 (linear mixed model with Benjamini-Hochberg false discovery rate correction). (B) Pictures of Go infected crks and close-up of infected leaves. In some cases, infected leaves displayed increased pigmentation and crk5 showed accelerated death of the infected leaves. Bar = 1 cm. (C) No infection was observed with the non-host powdery mildew Blumeria graminis f. sp. hordei (Bgh) but several crks displayed increased pigmentation. Bar = 1 cm. All experiments were repeated three times with similar results.