Fig_5.tif (495.18 kB)
Imaging of NTR activity in cells and in in vivo cancer model with NCL.
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posted on 2015-06-25, 03:40 authored by Anzhelika G. Vorobyeva, Michael Stanton, Aurélien Godinat, Kjetil B. Lund, Grigory G. Karateev, Kevin P. Francis, Elizabeth Allen, Juri G. Gelovani, Emmet McCormack, Mark Tangney, Elena A. Dubikovskaya(A) Concentration-dependent uncaging of NCL in MDA-MB-231 NTR+luc+ cancer cells in comparison with luciferin. (B) Selectivity of NTR imaging by NCL in the same cells in comparison with NTR-luc+ cells. The dashed line indicates background (cells only), *P = 0.0001. (C) In vivo imaging of NTR activity in subcutaneous NTR+ and NTR- xenografts (n = 5). Total luminescence over 1 h from IP injection of luciferin (1.5 mg) and NCL (1.9 mg). d) Representative images of mice 15 min post injection of luciferin or NCL.
