Identification of neural progenitor cell (NPC) markers in the rat FT.

a) Dissection of a P9 FT fixed in 4% formaldehyde. The arrow indicates the start of the FT. b) Immunohistochemical staining of a whole mount P3 FT for the NPC marker Nestin (red, i) with the distribution of cells indicated with DAPI (blue, ii). A merged image of b(i) and (ii) is shown in b(iii). Scale bar: 1 mm. c) Expression of Nestin (green) and DAPI (blue) in a longitudinal section of a P1 FT. Scale bar: 50 µm. d) Expression of Nestin (red) and DAPI (blue) in a transverse section of a P1 FT. Scale bar: 20 µm. The arrow in b(ii) indicates the location along the FT from which this section was taken.