ISA27 inhibits the growth/survival of GBM cell lines expressing wild-type p53.

GBM cells with wild-type p53 (U87MG, U343MG) were exposed to a range of ISA27 or Nutlin-3 concentrations for 24 h. After incubation, GBM cell viability was determined by MTS assay. A and B) Effect of ISA27 on the growth/survival of GBM cell lines expressing wild-type p53: curves for U87MG and U343MG cell samples were generated using a sigmoidal dose-response curve model (GraphPad Prism 4 software) from which the IC₅₀ values were derived. ISA27-treated cells (▾), Nutlin-3-treated cells (▪); C) Effect of ISA27 on the growth/survival of the GBM T98G cell line expressing mutant p53: T98G cells were incubated with increasing concentrations of ISA27 or Nutlin-3 (ranging from 1 µM to 25 µM), and cell viability was measured after 24 h by MTS assay. ISA27-treated cells (▾), Nutlin-3-treated cells (▪). The results are expressed as the percentages of cell viability with respect to the vehicle-treated sample for each cell line; the viability of the vehicle-treated sample was arbitrarily set at 100%. Points, means of three independent experiments performed in duplicate; bars, SEM.