ISA27 induces U87MG cell senescence.

<p><i>8A</i>) <i>Representative images of SA-β-Gal-expressing cells:</i> The panel shows the SA-β-Gal-expressing MDM2 inhibitor-treated and untreated cells at 72 h. <i>8B) Percentage of SA-β-Gal-expressing cells:</i> The number of SA-β-Gal-expressing cells was determined with respect to the total cells in each sample (untreated cells, ISA27- and Nutlin-3-treated cells). The percentage of SA-β-Gal-expressing MDM2 inhibitor-treated cells was then calculated with respect to the untreated cells, for which an arbitrary value of 100% was assigned.</p>