Figure_2.tif (1.19 MB)
ISA27 increases p53 protein levels in GBM cell lines.
figure
posted on 2013-08-19, 03:55 authored by Barbara Costa, Sara Bendinelli, Pamela Gabelloni, Eleonora Da Pozzo, Simona Daniele, Fabrizio Scatena, Renato Vanacore, Pietro Campiglia, Alessia Bertamino, Isabel Gomez-Monterrey, Daniela Sorriento, Carmine Del Giudice, Guido Iaccarino, Ettore Novellino, Claudia MartiniGBM cells (U87MG, U343MG) were treated with ISA27 or Nutlin-3 for the indicated incubation times, and protein levels of p53 were analysed in whole-cell lysates by Western blot. One representative Western blot is presented (upper panel) for each cell line. The blots show that the antibody to p53 (FL-393; Santa Cruz Biotechnology) recognised a single specific band at approximately 55 kDa, corresponding to the molecular weight of the p53 protein; β-actin is used as the loading control. Densitometric analyses of Western blots (lower panel) demonstrated that ISA27 induced a significant enhancement of p53 protein levels in U87MG or U343MG cells with a maximal effect at the 8 h incubation time.
