INO80 complex binds two p53 binding sites on the p21 promoter region in 293T cells.

(A) Six primer sets in the p21 locus used for amplifying ChIP’d DNA. (B) qPCR products from each primer set were subjected to 2.5% agarose gel and visualized by ethidium bromide. (C) Co-occupying of the INO80 complex and p53 at the -2.2kb and -1.0kb upstream of the p21 transcriptional start site. ChIP assays were performed using INO80, YY1, and p53 antibodies in 293T cells. ChIP’d DNA was analyzed by qPCR. Bar graphs show the ratios of ChIP’d DNA signals to IgG (all signals normalized to input). Error bars represent the standard error of the mean of 3 independent experiments. (D) Validation of the recruitment of the INO80 complex at the p21 promoter region. pBS-Vector and pBS-shINO80 transfected 293T cells (48 hours) were used in ChIP assays. ChIP’d DNA with INO80 and YY1 antibodies was analyzed by qPCR. Relative-fold enrichment vs IgG at the -2.2kb and -1.0kb upstream of the p21 transcriptional start site was displayed as bar graphs (all signals normalized to input). Error bars represent the standard error of the mean of 3 independent experiments.