IFN-β and ISG mRNA expression in neurotropic VSV infection.

Mice were infected intranasally with 5×10⁶ pfu of VSV or VSV-eGFP. A, Expression level of IFN-β in different brain parts was determined by real-time RT-PCR, A, IFN-β. B, Expression level of Mx2, Rsad2 USP-18 and IFIT-2 in the cerebrum was determined by real-time RT-PCR. Data represents mean with SEM of 3–7 mice in each group per time point. Asterisks indicate statistical significance calculated by Mann-Whitney test, ** p<0.005, * p<0.05. C, Representative pictures of immunohistological analysis of VSV-eGFP and IFIT-2 protein 6 days post infection in the cerebrum of IRF-1^−/− and WT mice. IFIT2 (red), DAPI (blue), VSV-eGFP (green). Scale bar is 50 μm.