Hypoxia maintains cell plasticity with a novel equilibrium of <i>Pluri</i> and <i>early Diff</i> genes.

<p>Cells grown under normoxia (N) or hypoxia (H) were depleted from LIF or induced with LIF after a period of LIF depletion as indicated. After 4 days, expression of the selected genes, (A) <i>Master</i>, (B) <i>Pluri</i>, (C) <i>Pluri Adhesion</i> and (D) <i>Diff</i> was analysed by RT-qPCR. Graphs represent the average level of expression and SEM as depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146281#pone.0146281.g002" target="_blank">Fig 2</a> (n = 4). One sample t-test was performed for the +LIF condition and paired sample t-test was performed for the other conditions to evaluate the significance of the difference in the expression levels observed in normoxia versus hypoxia: *p-value<0.05; **p-value<0.01, ***p-value<0.001; if not stated: not significant.</p>