Hr-anxA1 does not affect macrophage polarization in atherosclerotic plaque.

(A) Representative staining for M1 macrophage specific marker iNOS. Black arrows indicate fully commited M1 macrophages, white arrows indicate iNOS negative macrophages. (B) Quantification of iNOS-staining indicates no difference between control and anxA1 treated animals. (C) Representative staining of M2 specific marker Ym1. Both control and hr-anxA1 treated mice were negative for Ym1. (D) Staining of Ym1 positive cells in the spleen indicating functionality of anti-Ym1 antibody. All values are represented as mean ±SEM, n = 12 animals per group. Panel A: 200x magnification, scale bar represents 100μm; panel D: 40x and 400x magnification, scale bar represent 500μm and 50μm respectively.