Histological features of the skin of Nc/Nga, Balb/c and C57Bl/6 mice 7 days after mock or WR inoculation by vaccination stamp.
Mock- (A, C) and OVA-sensitized (B, D) mice were mock-inoculated with OVA in PBS (A, B) or inoculated with 107 PFU of a purified stock of WR with OVA in PBS (C, D). Hematoxylin-eosin stained skin sections. Original magnification: (A, B) upper panel 100x, lower panel 400x; (C, D) upper panel 100x, lower panel 200x with inserts 400x. (A) Nc/Nga - Mild mixed predominantly mononuclear dermal inflammatory infiltrate. Thickening of epidermis, increased thickness of dermis due to proliferation of the connective tissue. Balb/c - Mild fibrosis and no or minimal mixed inflammation in the dermis. C57Bl/6 - Minimal mixed dermal inflammatory infiltrate in the skin, focal thickening of epidermis. (B) Nc/Nga – Very mild mixed dermal inflammatory infiltrate. Thickening of epidermis and parakeratosis (↓), increased thickness of dermis. Balb/c - Thickening of epidermis, mild fibrosis and mild mixed dermal inflammation. C57Bl/6 - Focal thickening of epidermis, very mild mixed dermal inflammatory infiltrate. (C, D) WR-inoculated mice. In all strains - mixed dermal inflammatory infiltrate with an epidermal erosion/ulcer, covered by a crust. Florid necrotizing folliculitis with high incidence of chromatin debris →, eosinophils found in Nc/Nga, Balb/c and C57Bl/6 mice in about 5, 1 and 3%, respectively – shown in the inserts. Representative results of skin sections of 3-5 animals.
