Hematopoietic stem/progenitor cells decreases in the fetal liver of CALM^−/− mice and their colony forming activities are impaired.

(A) Fetal liver cells were harvested from wild-type (WT) (indicated +/+), CALM^+/− (indicated +/−), and CALM^−/− (indicated −/−) mice on embryonic day 14.5 (E14.5). Numbers of mononuclear cells (MNCs) in each fetal liver were calculated by cell counter. The proportion of Linage⁻Sca-1⁺KIT⁺ (LSK) cells in MNCs was determined by flow cytometry. Data represent means ± SD (n = 6, *p<0.05). (B) LSK cells isolated from fetal liver of WT, CALM^+/−, and CALM^−/− mice on E14.5 were subjected to colony assays. All cultures were conducted in triplicate and number of colonies were counted after 14 days. Results are shown as mean ± SD (n = 6, *p<0.05).