Hch1p regulates Hsp90 inhibitor drug sensitivity in yeast.

A. Deletion of HCH1 confers resistance to Hsp90 inhibitor NVP-AUY922 in yeast expressing Hsp82p, Hsp82pG313S or Hsp82pA587T. Cells were grown overnight in YPD and then diluted to 1×108 cells per mL. 10-fold serial dilutions were prepared and 5 µL aliquots were spotted on YPD agar plates supplemented with indicated concentrations of NVP-AUY922. B. Overexpression of myc-tagged Hch1p results in hypersensitivity to Hsp90 inhibitor NVP-AUY922 in yeast. Cells were grown overnight in YPD supplemented with 200 mg/L G418 and then diluted to 1×108 cells per mL. 10-fold serial dilutions were prepared and 5 µL aliquots were spotted on YPD agar plates supplemented with 200 mg/L G418 and indicated concentrations of NVP-AUY922.