HSAF sensitivity of ΔBAR1, ΔGCS1, and ΔMAPK mutants.

A. ΔBAR1 mutants are resistant to HSAF. 7 µl of macroconidial suspensions (serially diluted from 105 to 102 per ml) were spotted onto plates containing different concentrations of HSAF. B. MAPK mutant ΔMGV1 is hypersensitive to HSAF, but ΔGPMK1 is unchanged in sensitivity. C. Glucosylceramides contribute to HSAF sensitivity, as a Yarrowia lipolytica ΔGCS1 mutant is resistant to HSAF. Plates in panels B and C were inoculated as described in A.