HPTLC-immunostaining of brain gangliosides, such as GM1, GD1a, GD1b, GT1b, and GQ1bα (plate A) and GM1, GD3, GQ1b, and SGPG (plate B).

Lane 1, 1 µg each of GM1, GD1a, GD1b, and GT1b; 2, 1 µg of GM1 and GQ1bα; 3, 1 µg each of GM1, GD3, and GQ1b; and 4, 1 µg of SGPG. a, orcinol sulfuric acid staining; b, c, and d, immunostaining; b-1 and b-2, control sera; c-1 and c-2, AD sera (Alzheimer’s disease); d-1 and d-2, VD sera (vascular dementia). Plates A and B were developed with CH₃Cl₃:CH₃OH:0.2%CaC₂•2H₂O (55∶45:10, v/v).