Figure_2.tif (245.25 kB)
HPLC chromatograms of the metabolite of nifedipine (NIF) catalyzed by recombinant CYP3A29.
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posted on 2014-09-03, 02:59 authored by Guyue Cheng, Changcun Liu, Xu Wang, Hongmin Ma, Yuanhu Pan, Lingli Huang, Haihong Hao, Menghong Dai, Zonghui Yuan(A) NIF standard. (B) Oxidized nifedipine (ONIF) standard. (C) NIF incubated with Sf9 microsomes containing the recombinant CYP3A29 at 37°C for 30 min in the presence of NADPH-generating system. (D) NIF incubated with Sf9 microsomes free of the recombinant CYP3A29 at 37°C for 30 min in the presence of NADPH-generating system. (E) NIF incubated with Sf9 microsomes containing the recombinant CYP3A29 at 37°C for 30 min in the absence of NADPH-generating system.
