HPLC analysis of tocopherols and tocotrienols.
(A) HPLC chromatograms of reference standards. Peak 1, δ-tocotrienol; Peak 2, γ-tocotrienol; Peak 3, α-tocotrienol; Peak 4, δ-tocopherol; Peak 5, γ-tocopherol; Peak 6, α-tocopherol. (B) HPLC chromatograms of extracts from the leaves of ZH11 (top) and lyl1-1 (bottom), respectively. (C) The contents of α-tocopherol in ZH11 and lyl1-1 plants. (D) HPLC chromatograms of extracts from the leaves of Nipp (top) and lyl1-2 (bottom), respectively. (E) The contents of α-tocopherol in Nipp and lyl1-2 plants. The tocopherols were extracted from the first, second and third leaves of ZH11, lyl1-1, Nipp and lyl1-2 plants grown under natural conditions (high light). Data presented are mean ±SD. ** Significant at the 0.01 level.