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HPLC analysis of tocopherols and tocotrienols.

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posted on 2013-09-10, 01:35 authored by Yong Zhou, Zhiyun Gong, Zefeng Yang, Yuan Yuan, Jinyan Zhu, Man Wang, Fuhai Yuan, Shujun Wu, Zhiqin Wang, Chuandeng Yi, Tinghua Xu, MyongChol Ryom, Minghong Gu, Guohua Liang

(A) HPLC chromatograms of reference standards. Peak 1, δ-tocotrienol; Peak 2, γ-tocotrienol; Peak 3, α-tocotrienol; Peak 4, δ-tocopherol; Peak 5, γ-tocopherol; Peak 6, α-tocopherol. (B) HPLC chromatograms of extracts from the leaves of ZH11 (top) and lyl1-1 (bottom), respectively. (C) The contents of α-tocopherol in ZH11 and lyl1-1 plants. (D) HPLC chromatograms of extracts from the leaves of Nipp (top) and lyl1-2 (bottom), respectively. (E) The contents of α-tocopherol in Nipp and lyl1-2 plants. The tocopherols were extracted from the first, second and third leaves of ZH11, lyl1-1, Nipp and lyl1-2 plants grown under natural conditions (high light). Data presented are mean ±SD. ** Significant at the 0.01 level.

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