HNPCs cultured on top of BS slices form honeycomb-like networks and primarily neurons.

A. Distribution of HNPCs detected by h nuc (red)- or GFP (green)- expression was uniform throughout the entire surface of the BS slice. GFAP (white)- expression was mainly seen in the center of the human cell mass. B. GFP (green)/GFAP (white)-labelling revealed large numbers of HNPC processes expressing GFAP. C. Neuronal differentiation was confirmed by Tuj1 (white)-staining co-localizing with h nuc (red) and GFP (green). Arrowhead in insert shows GFP/Tuj1+ process. D. Neuronal differentiation was also confirmed by DCX (red)/GFP+ processes (arrowheads in insert). E. A honeycomb-like pattern was formed by GFP+ HNPCs network on the surface of the BS slice. F. The vast majority of GFP-labeled cells had morphologies of migrating/differentiating neurons (arrowheads). Scale bar equals 200 µm in A, 100 µm in B; 200 µm in C, D; 50 µm in E, 100 µm in F.