Figure_5.tif (140.85 kB)
HIV-1 proteins enhance LPS-S activity in induction of CXCR3-associated chemokine by human hepatocytes.
figure
posted on 2014-02-06, 09:53 authored by Sishun Hu, Marwan Ghabril, Tohti Amet, Ningjie Hu, Daniel Byrd, Kai Yang, Raj Vuppalanchi, Romil Saxena, Mona Desai, Jie Lan, Raymond Johnson, Samir Gupta, Naga Chalasani, Qigui YuHuh7.5.1 cells in 24-well plates were treated with HIV-1 gp120 (1.0 µg/mL) or Tat (1.0 µg/mL) in the presence or absence of LPS-S from LPS-stimulated PBMCs (1∶10 dilution) for 24 h. After stimulation, cell-free supernatant was harvested and then subjected to an ELISA assay to determine protein levels of CXCL9, CXCL10, and CXCL11. Horizontal bars represent mean ± SD of chemokine protein levels from triple wells of each dose. Note that different Y-axis values among CXCL9, CXCL10 and CXCL11 are used. p<0.05 indicates significant differences. NS, not significant, **, p<0.01; and *, p<0.05.
