Growth of the wild type (WT) and knockout (KO) in PBS (0) or 10, 20, 30, 40, or 50% of human serum diluted in PBS
(A). Susceptibility test of WT, HET, and RE strains with indicated concentrations of cerulenin (µg/ml) in YPD agar incubated at 30°C for 2 days (B). Inhibition of WT growth by indicated concentration of cerulenin in 50% human serum (C). The number of CFU was expressed as log10. Experiments were repeated twice with triplicates. Error bars indicate standard deviation. *P<0.01; **P<0.05 (ANOVA).