Growth of the <i>pmr1-Δ</i> and <i>ret1-27</i> mutants on SD* medium supplemented with different concentrations of MnCl<sub>2</sub>.

<p>Cell suspensions with equal densities were spotted onto corresponding media and grown for 2 days. The experiment was performed using serial dilutions of cell suspensions (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145915#pone.0145915.s003" target="_blank">S3 Fig</a>) and a representative dilution is shown in this figure. <i>pmr1-Δ</i>, a subclone of the 1MA27/12/GP1 strain lacking the <i>PMR1</i> containing plasmid; the <i>PMR1</i>, 1MA27/12/GP1 strain, <i>ret1-27</i>, the 64MA70QAL strain; <i>RET1</i>, the 64MA70QA-RET strain.</p>