Gi coupling of MT1 reconstituted in lipid nanodiscs.

Kinetics of GTPγS binding to Gi in the Gαiβ1γ2 trimer triggered by MT1R-containing nanodiscs in the absence of ligand (open circles) or in the presence of 10 µM of the melatonin agonist (closed circles). The kinetics measured in the presence of empty nanodiscs is given as open squares. These data were treated as described in the Materials and Methods section to extract the basal exchange rates.