Germ cell apoptosis is elevated in <i>syp-4</i> mutants.

<p>acontrol RNAi with empty pL4440 vector. Germ cell corpses were scored in adult hermaphrodites 20 hours post-L4 as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000669#pgen.1000669-Kelly1" target="_blank">[59]</a>. Statistical comparisons between genotypes were conducted using the two-tailed Mann-Whitney test. <i>syp-4(tm2713)</i> (n = 57; where n = number of gonad arms scored/genotype) differed significantly from wild type (n = 42) (p<0.0001), and from <i>spo-11</i>(n = 17) (p<0.0001), but did not differ significantly from <i>syp-3(ok758)</i> (n = 22) (p = 0.8471). <i>syp-4(RNAi);spo-11/nT1</i> (n = 51) differed significantly from <i>syp-4(RNAi);spo-11/spo-11</i> (n = 58) (p = 0.0122), <i>spo-11/nT1</i>(n = 20) (p<0.0001), and <i>spo-11/spo-11</i>(n = 17) (p = 0.0016). <i>syp-4(RNAi);spo-11/spo-11</i> and <i>spo-11/spo-11</i>(n = 17) did not differ significantly from each other (p = 0.0905).</p>