Germ cell apoptosis is elevated in syp-4 mutants.

acontrol RNAi with empty pL4440 vector. Germ cell corpses were scored in adult hermaphrodites 20 hours post-L4 as in [59]. Statistical comparisons between genotypes were conducted using the two-tailed Mann-Whitney test. syp-4(tm2713) (n = 57; where n = number of gonad arms scored/genotype) differed significantly from wild type (n = 42) (p<0.0001), and from spo-11(n = 17) (p<0.0001), but did not differ significantly from syp-3(ok758) (n = 22) (p = 0.8471). syp-4(RNAi);spo-11/nT1 (n = 51) differed significantly from syp-4(RNAi);spo-11/spo-11 (n = 58) (p = 0.0122), spo-11/nT1(n = 20) (p<0.0001), and spo-11/spo-11(n = 17) (p = 0.0016). syp-4(RNAi);spo-11/spo-11 and spo-11/spo-11(n = 17) did not differ significantly from each other (p = 0.0905).