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Genes in the region of the igaB insertion site.
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posted on 2013-02-20, 14:05 authored by Timothy F. Murphy, Alan J. Lesse, Charmaine Kirkham, Huachun Zhong, Sanjay Sethi, Robert S. Munson JrA. Map of genes from ORFs HI0163 through HI0185 (not to scale) in H. influenzae strain Rd KW20 which does not contain the igaB gene. The dotted line represents ORFs HI0167 through HI0182. Horizontal arrows indicate location of oligonucleotide primers used in PCR reactions. B. Ethidium bromide stained agarose gels showing results of PCR reactions with genomic DNA of H. influenzae strains that do not contain the igaB gene with the oligonucleotide primers noted in A. Lanes contain genomic DNA from strains a. Rd KW20, b. 11P6H (which contains igaB), c. 5P28H1, d. 14P14H1, e. 31P7H1, f. 32P8H1, g. 48P28H1, h. 56P76H1, i. 1862, j. 3222B. Molecular size standards are noted in kilobases.
