Generation of BALB/cByJ-<i>CD11B</i>-<i>Kif1c<sup>D2</sup></i> transgenic (Tg-<i>Kif1c<sup>D2</sup></i>) mice.

<p>(A) Schematic representation of the <i>Kif1c</i> gene used to generate the transgenic mice showing the promoter (<i>CD11B/ITGAM</i>), and the <i>Kif1c</i> gene, followed by the hGH/polyA signal sequence. Arrows indicate PCR-primers for screening. (B and C) Percentage of splenic F4/80<sup>+</sup> (B) and CD11b<sup>+</sup> (C) cells of Tg-<i>Kif1c<sup>D2</sup></i> and NLC. The analysis was performed on gated live cells according to their FSC vs. SSC profile. Statistical significance was determined using the Mann-Whitney <i>U</i> test. Data represent the mean ± SEM of at least 5 individual mice. (D) Kif1c expression in thioglycolate-induced adherent cells by Western blotting using whole-cell extracts and the anti-Kif1c mAb. Actin was used as a loading marker. (E) mRNA expression of <i>Kif1c</i> was measured from sorted TCRβ<sup>−</sup>CD19<sup>−</sup>CD11b<sup>+</sup> myeloid cells of CByJ mice and compared with TCRβ<sup>−</sup>CD19<sup>−</sup>CD11b<sup>+</sup> myeloid cells of D2 mice. β2-microglobulin and GAPDH were used as an endogenous control. Data represent the mean ± SEM of two experiments (pool of 5 animals/each).</p>