Gene expression of glycolytic transcription factors in overexpression (OE) or knockout (KO) HAP5 yeast strains.

Yeast cells were cultured in a YPD medium (A, B, C) or YPD with 30 µg/ml neomycin (D, E, F) for 16 h and harvested. RNA was then extracted for quantitative RT-PCR analyses. The relative gene expression of RAP1 (A, D), GCR1 (B, E) and GCR2 (C, F) of strains was normalized to the average content per cell of ACT1. Values are expressed as percentages of the average values for the wild-type strain MSS1(P^S) in YPD medium. Samples from at least three independent cultures of RNA content and ACT1 for each strain were used in the calculations. *P<0.05, **P<0.01.