Gender difference in TAC-induced LV CaMKP expression and phosphatase activity.
2014-03-07T03:07:33Z (GMT) by
<p>(<b>A</b>) Immunoblots from mouse hearts of both genders 30 days after sham-operation. (<b>B</b>) Densitometric analysis of Western blot of CaMKP protein levels normalized to GAPDH. At basal level, CaMKP protein level was higher in females (F Sham) than in males (M sham). (<b>C</b>) Immunoblots from mouse hearts of both genders 30 days after sham-operation (Sham) or thoracic aortic constriction (TAC). (<b>5D</b>) Densitometric analysis of Western blots of CaMKP in TAC mice of both genders. CaMKP protein levels in TAC mice are expressed as fold changes relative to their respective shams. (<b>E</b>) In-agarose gel detection of phosphatase activity, using 4-MUP as substrate, of CaMKP immunoprecipitated with anti-CaMKP antibodies from mouse LVs of both genders (left panel: male mice; right panel female mice). RP: CaMKP recombinant protein. (<b>F</b>) Phosphatase activity of IP-CaMKP was assayed using para-nitrophenyl phosphate as substrate. *p<0.05, TAC <i>vs.</i> sham; <sup>#</sup> p<0.05, female <i>vs.</i> male.</p>