Gender difference in TAC-induced LV CaMKP expression and phosphatase activity.
(A) Immunoblots from mouse hearts of both genders 30 days after sham-operation. (B) Densitometric analysis of Western blot of CaMKP protein levels normalized to GAPDH. At basal level, CaMKP protein level was higher in females (F Sham) than in males (M sham). (C) Immunoblots from mouse hearts of both genders 30 days after sham-operation (Sham) or thoracic aortic constriction (TAC). (5D) Densitometric analysis of Western blots of CaMKP in TAC mice of both genders. CaMKP protein levels in TAC mice are expressed as fold changes relative to their respective shams. (E) In-agarose gel detection of phosphatase activity, using 4-MUP as substrate, of CaMKP immunoprecipitated with anti-CaMKP antibodies from mouse LVs of both genders (left panel: male mice; right panel female mice). RP: CaMKP recombinant protein. (F) Phosphatase activity of IP-CaMKP was assayed using para-nitrophenyl phosphate as substrate. *p<0.05, TAC vs. sham; # p<0.05, female vs. male.