GSK669 exhibits NOD2 selectivity in cells that express endogenous NOD1 and NOD2.

<p>Concentration response curves of GSK669 and its close analogue GSK400 for the inhibition of MDP (A) but not Tri-DAP (B) stimulated IL-8 secretion in HCT116 cells which express functionally active NOD1 and NOD2. Cells were pre-incubated with compounds for 1 hour prior to addition of NOD agonists. IL-8 secreted into medium was assayed after 24 hours. The same RIP2 inhibitor used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069619#pone-0069619-g002" target="_blank">Figure 2C</a> was included as a positive control. Data are the average percent inhibition obtained from 1 (RIP2), 2 (GSK400) or 4 (GSK669) separate experiments.</p>