GSK669 exhibits NOD2 selectivity in cells that express endogenous NOD1 and NOD2.
Concentration response curves of GSK669 and its close analogue GSK400 for the inhibition of MDP (A) but not Tri-DAP (B) stimulated IL-8 secretion in HCT116 cells which express functionally active NOD1 and NOD2. Cells were pre-incubated with compounds for 1 hour prior to addition of NOD agonists. IL-8 secreted into medium was assayed after 24 hours. The same RIP2 inhibitor used in Figure 2C was included as a positive control. Data are the average percent inhibition obtained from 1 (RIP2), 2 (GSK400) or 4 (GSK669) separate experiments.