Figure_5.tif (1.11 MB)

GLI suppression does not induce a luminal-like phenotype in androgen-independent cells.

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posted on 2011-05-25, 00:40 authored by Sandeep K. Nadendla, Allon Hazan, Matt Ward, Lisa J. Harper, Karwan Moutasim, Lucia S. Bianchi, Mahmoud Naase, Lucy Ghali, Gareth J. Thomas, David M. Prowse, Michael P. Philpott, Graham W. Neill

(A) The transformed morphology of LNCaP-GLI1 cells does not reverse upon transfection with GLI1 or GLI2 siRNA. (B) qPCR analysis of GLI1 and GLI2 mRNA in LNCaP-GLI1 cells transfected with GLI1 or GLI2 siRNA. (C) RT-PCR analysis of ΔNp63 and AR mRNA in LNCaP-GLI1 cells transfected with GLI1 or GLI2 siRNA. (D) qPCR analysis of GLI1 and GLI2 mRNA in DU145 and PC-3 cells transfected with GLI1 and GLI2 siRNA. (E) GLI reporter activity is suppressed in DU145 and PC-3 cells transfected with GLI1 and GLI2 siRNA (n.b. reporter activity may be influenced by GLI3 expression in PC-3 cells [17]). (F) RT-PCR analysis of ΔNp63 and AR mRNA in DU145 and PC-3 cells transfected with GLI1 and GLI2 siRNA.