Functionality of exogenous CD46 in MP₃₀CD46-transduced CHO cells. (A), CD46 as complement C3 regulator.

CHO cells were harvested at 48 h posttransfer, and the CD46-induced protection against complement C3-mediated cell apoptosis was assayed by the percentage of Annexin V-positive cells determined by flow cytometry at increasing doses of complement C3. (B), Kinetics of the gain of adenoviral receptor function by MP₃₀CD46-transduced CHO cells. CHO cells were harvested at different times after MP₃₀CD46-transfer, and cell permissiveness to the HAdV5F35-GFP vector was assessed by infection with HAdV5F35-GFP at MOI 500. Cells were analyzed for GFP signal at 48 h postinfection. The degree of permissiveness to the vector was expressed as the percentage of GFP-positive cells (left y-axis), and the relative transduction efficiency (RTE; right y-axis). The RTE, in arbitrary units (AU), was given using the formula = (percentage of GFP-positive cells) x (MFI; mean fluorescence intensity).