Functional and physical link between CD9P-1 and α2β1 integrin.

(A) Time-lapse videomicroscopy experiments were performed with HEK-293 or HEK/CD9P-1 cells. Monoclonal antibodies directed against integrins α1 (FB12) or α2 (Gi9) were added in the media (10 µg/ml). Independent experiments were performed (n = 6); standard errors are shown; (*) p<0.01. (B) Cells were lysed using the mild detergent Brij58 and immunoprecipitation experiments were performed with mAbs directed against CD9P-1 (1F11), α2-integrin (Gi9), β1-integrin (9D6) and CD55. After electrophoresis and transfer to a membrane, Western blotting was performed using α2-integrin antibody H-293.