Figure_7.tif (835.56 kB)
Frequency of JR-FL Env-specific CD4+ T cell responses in the lung and spleen.
figure
posted on 2014-09-12, 02:51 authored by Svetlana Rabinovich, Rebecca L. R. Powell, Ross W. B. Lindsay, Maoli Yuan, Alexei Carpov, Aaron Wilson, Mary Lopez, John W. Coleman, Denise Wagner, Palka Sharma, Marina Kemelman, Kevin J. Wright, John P. Seabrook, Heather Arendt, Jennifer Martinez, Joanne DeStefano, Maria J. Chiuchiolo, Christopher L. Parks1.5×106 leukocytes isolated from the lungs (a, b) andspleen (c, d) at the end of the study were stimulated ex vivo with JR-FL gp140, anti-CD28 and brefeldin A before being analyzed by flow cytometry for production of IFNg, IL-2 and TNFa. The total frequency of cytokine secreting CD4+ T cells (%) producing IFNγ, IL-2 or TNFα are shown on the left (a, c) and the frequency of multifunctional CD4+ T cells producing any combination of IFN γ +, IL-2+, and/or TNFα+ on the right (b, d). *p<0.05 compared to group 1. #p<0.05 compared to group 2. ∧p<0.05 compared to group 4. ∇p<0.05 compared to group 5. @p<0.05 compared to group 6. xp<0.05 compared to group 7. Bars represent the median (with SEM), individual animals are shown.
History
Usage metrics
Categories
Keywords
G positionSignificant quantitiesimmunogenic HIVConformationally Intacthumoral responsesnovel vectorcytoplasmic tail regionssurface glycoproteinG generegimen elicitingFunctional HIVflow cytometryEnvG functionalityIM VSVsurface EnvGEnv trimersHIV vaccine platformclade B Env immunogenattenuating quantitiesSIV challengestomatitis virusgrowth attenuationelisaattenuated HIVHIV immunogensElicits Potent CellularEnvelope TrimersWestern Blot analysissurface Gilrhesus macaquesneutralizing Ab titersIM electroporated plasmids encoding EnvGsafety concerns
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC