Figure_5.tif (4.24 MB)
Fluorescence staining of MG 63 cells with a LIVE/DEAD viability/cytotoxicity kit (Invitrogen, Molecular Probes, U.S.A.) on day 7 after seeding on a microscopic glass coverslip (A) standard polystyrene cell culture dish (B), undoped NCD films (C), NCD films doped with boron in concentrations of 133 ppm (D) 1000 ppm (E) and 6700 ppm (F).
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posted on 2013-02-20, 17:41 authored by Lubica Grausova, Alexander Kromka, Zuzana Burdikova, Adam Eckhardt, Bohuslav Rezek, Jiri Vacik, Ken Haenen, Vera Lisa, Lucie BacakovaViable cells are stained in green with calcein, dead or damaged cells in red with ethidium homodimer-1. Olympus IX 51 epifluorescence microscope, DP 70 digital camera, obj. 20×, bar = 100 µm.
