Fluorescence spectra of TNP-ATP bound to <i>M</i>. <i>leprae</i> HSP18.

<p><b>(A)</b> Fluorescence emission spectra of TNP-ATP alone (trace 1); TNP-ATP+HSP18 (trace 2); Addition of 0.25 mM ATP to the HSP18-TNP-ATP complex (trace 3). Protein concentration used was 2.5 μM and TNP-ATP concentration was 2.5 μM. Excitation wavelength used was 403 nm and fluorescence emission spectra were recorded from 500–600 nm. <b>(B)</b> Fluorescence titration of 2.5 μM <i>M</i>. <i>leprae</i> HSP18 was carried with varying concentration of TNP-ATP with fluorescence monitored at λ<sub>exc</sub> = 403 nm and λ<sub>em</sub> = 538 nm.</p>