Fluorescence of ofloxacin in PBS (dot), ofloxacin in rabbit serum (black solid), and rabbit serum without ofloxacin (gray solid).

Ofloxacin concentrations in PBS and serum were respectively 140 and 212 µmom/L (51 and 76 mg/L). Serum had a high intrinsic autofluorescence at 340 nm.