Flow cytometry assessment of MAPK phosphorylation in whole blood samples of SR and SS asthma patients.

Whole blood aliquots were stained with either anti-CD3, CD4, CD8 antibody cocktail or CD14, CD16, CD20 antibody cocktail plus one of the phospho-MAPK antibodies (i.e. p-p38, p-ERK, p-JNK) or corresponding IgG isotype control antibodies (to detect nonspecific antibody binding). (A) Phosphorylation of p38 in CD14⁺ monocytes of subjects with SR asthma vs. SS asthma (MFI). (B) Phosphorylation of ERK in CD14+ cells of subjects with SR asthma vs. SS asthma (MFI).