Figure_1.tif (923.01 kB)
Flow cytometric seed screen on single seeds of Boechera gunnisoniana to analyse ploidy.
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posted on 2014-07-10, 03:35 authored by Anja Schmidt, Marc W. Schmid, Ulrich C. Klostermeier, Weihong Qi, Daniela Guthörl, Christian Sailer, Manuel Waller, Philip Rosenstiel, Ueli GrossniklausFluorescence intensity was measured on individual green seeds of Boechera to determine the ploidy level of embryo and endosperm. 98% of the seeds measured (N = 84) showed a 3C∶9C ratio of embryo∶endosperm, indicating diplosporous apomixis (A). The remaining 2% showed a 6C∶9C embryo∶endosperm ratio indicative of a BIII hybrid where the embryo is derived from fertilization of an unreduced egg cell (B).
