Fine-mapping of the QTL for hard seededness (qHS1).
A segregating family of a backcross inbred line derived from a cross between Tachinagaha and a wild soybean accession was used for fine-mapping. Graphical genotypes are presented for plants (B5F4) with recombination in the genomic region harboring the QTL (A) and their progeny (B5F6) homozygous for recombinant genotypes (B). Open, dark gray, and black bars indicate regions homozygous for the allele from Tachinagaha (TA allele), heterozygous, and homozygous for the allele from the wild soybean (AO allele), respectively. Light gray bars indicate a region in which recombination occurred. Phenotypes for permeability are presented at right. In (A), the permeability of the B5F4 plants was evaluated based on the results of a progeny test (Seg, segregating for permeability; Fixed, homozygous for permeability). In (B), HS (%) indicates percent of hard seeds. C, The delineated region of 93 kb included 10 annotated genes in the Williams 82 genome sequence database (Phytozome Glyma1.0). D, Mutation site in the endo-1,4-β-glucanase gene (Glyma02g43680) and predicted amino acid sequences. Underline, PvuII restriction site.
