Figure_1.tif (1.08 MB)
Filovirus species-specificity of IgG antibodies detected in orangutan sera.
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posted on 2012-07-18, 02:12 authored by Chairul A. Nidom, Eri Nakayama, Reviany V. Nidom, Mohamad Y. Alamudi, Syafril Daulay, Indi N. L. P. Dharmayanti, Yoes P. Dachlan, Mohamad Amin, Manabu Igarashi, Hiroko Miyamoto, Reiko Yoshida, Ayato TakadaSerum samples diluted 1∶100 were tested for IgG antibodies reacting with soluble GP antigens in ELISA (A) and with GP, NP, and VP40 in Western blotting (B). Representative data for ZEBOV (#307), SEBOV (#116), CIEBOV (#340), BEBOV (#304), REBOV (#182), MARV (#106), and multiple (#214) GP-positive samples are shown. Asterisks indicate significantly higher OD values determined by the Smirnov-Grubbs rejection test (P < 0.01). VLPs consisting of GP, NP, and VP40 were used for Western blotting, and rabbit and mouse antisera or mouse monoclonal antibodies ZGP43/3.7 and AGP127-8 was used as positive controls as described in Materials and Methods. 1, ZEBOV; 2, SEBOV; 3, CIEBOV; 4, BEBOV; 5, REBOV; 6, MARV; 7, negative control.
