Figure 4

<p>. <b>Overlapping RNA fragments covering the entire genome of HIV-1 </b><b><i>hxb2</i></b><b>.</b> PCR fragments of approximately 500 bp, with overlaps of 250 bp, and covering the entire genome of HIV-1 <i>hxb2</i> were <i>in vitro</i> transcribed with T7 RNA polymerase into a set of uncapped and unpolyadenylated RNA fragments. After purification RNA fragments were migrated on Agilent-2100 Small RNA chips. A ladder on the left indicates the size in nucleotides.</p>