FPLC-Western Blot and Mass spectroscopy screening of auto-Ab candidates.

A) Control human brain protein content was fractioned by molecular weight using FPLC. Fractions between 170 and 50 kDa are shown on a polyacrilamide gel (15% SDS) to further separate proteins by molecular weight. This two-step technique allows for a comprehensive separation of the total brain protein content. B) A parallel gel was run and processed for western blot analysis with player's serum (highest measured auto-S100B antibody, # in Figure 4) used as a “primary” antibody. Bands of interest (double asterisks) were excised and processed for MS analysis. Results are shown in the Table (C) and described in the text.