posted on 2015-07-31, 03:26authored byKaisa Tamminen, Diego Balboa, Sanna Toivonen, Mikko P. Pakarinen, Zoltan Wiener, Kari Alitalo, Timo Otonkoski
A. Expression of hepatic markers AFP (n = 4–15) and ALB (n = 4–14) during hindgut differentiation induced by different concentrations (ng/ml) of WNT3A (W) and FGF4 (F) and 3 μM CHIR99021 (mean ± SEM). B. Double immunocytochemistry for CDX2 and AFP at day 9. C. Expression levels of AFP and ALB during differentiation with varying concentrations of CHIR, (mean ± SEM; n = 3). D. Immunocytochemistry for VIM at day 9. Scale bars 100 μm.