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FBXO44-Mediated Degradation of RGS2 Protein Uniquely Depends on a Cullin 4B/DDB1 Complex - Fig 3
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posted on 2015-05-13, 02:47 authored by Benita Sjögren, Steven Swaney, Richard R. NeubigA. FLAG-FBXO44 co-immunoprecipitates with RGS2-HA. B. The reciprocal experiment shows that RGS2-HA co-immunoprecipitates with FLAG-FBXO44. Also note that co-expression with FLAG-FBXO44 reduces total protein levels of RGS2-HA. C. RGS4C2S-HA does not co-immunoprecipitate FLAG-FBXO44. The RGS4C2S-HA mutant was used due to the low protein expression obtained with wild-type RGS4-HA. D. Schematic of full length FBXO44 and FBXO44ΔN. E. RGS2-HA co-immunoprecipitates both full length FBXO44 and FBXO44ΔN. F. RGS2 immunoprecipitates with FBXO44 in lysates from mouse whole heart tissue.
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cullin 4 BSkp 1genomic siRNA screening approachprotein degradationproteasomal degradationRGS 2 degradationmediates RGS 2 protein degradationE 3 ligasecell functionCUL 4B DNA damage binding protein 1DDBRGS 2 dysregulationnovel E 3 ligaseFBXObind RGS 2RGS 2 Protein Uniquely DependsG Protein Signaling 2
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