Figure_1.tif (1.31 MB)
FACs sorting of reticulocytes and extruded nuclei.
figure
posted on 2013-04-03, 06:21 authored by Amanda J. Bell, Timothy J. Satchwell, Kate J. Heesom, Bethan R. Hawley, Sabine Kupzig, Matthew Hazell, Rosey Mushens, Andrew Herman, Ashley M. ToyeA) Extruded nuclei and reticulocytes were separated by fluorescence activated cell sorting based on fluorescence intensity of DNA (Hoechst) and GPA (BRIC256) staining as outlined in the Materials and Methods. B) Representative cytospins from the sorted reticulocyte (upper panel) and extruded nuclei (lower panel) populations are shown.
