Extremes of gating phenotypes of cysteine-substituted S2D4 mutants, F1385C and E1392C compared with wild-type channels.

2009-11-02T02:17:44Z (GMT) by Zhongming Ma Jun Kong Roland G. Kallen
<p><b>A</b>, Family of currents recorded from tsA201 cells expressing WT (left) and the most positively (F1385C, middle) and negatively perturbed mutant channels (E1392C, right) in terms of h<sub>∞</sub> (see 1C below). Current families were elicited by voltage pulses from a holding potential of −120 mV rendered in 5 mV increments. <b>B</b>, Current-voltage (G–V) relations for WT and mutant channels (E1392C, F1385C) in A. <b>C</b>, Availability-voltage (h<sub>∞</sub>-V) relations for WT, F1385C (largest hyperpolarizing shift) and E1392C, (largest depolarizing shift). Symbols are normalized (I/I<sub>max</sub>) values and solid lines are single Boltzmann fits to the data (most significant parameter perturbations contained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007674#pone-0007674-t001" target="_blank">Table 1</a> the values for the entire set of mutant channels are contained in the Table A, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007674#pone.0007674.s001" target="_blank">Appendix S1</a>)). <b>D</b>, Current decay time constants (τ<sub>h</sub>). <b>E</b>, Recovery from inactivation time constants (τ<sub>rec</sub>). The values for τ<sub>h</sub> and τ<sub>rec</sub> for the entire set of mutant channels are contained in the Table B <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007674#pone.0007674.s001" target="_blank">Appendix S1</a>.</p>