Expression profiles of NLRP and PRAME family genes in human and mouse.
Most NLRP family members in the human microarray data share maternal expression pattern or are expressed at low levels (A). Mouse NLRP family orthologs in the TaqMan array share the similar maternal expression pattern, except for Nlrp12 (B). One gene in the mouse PRAME family is maternally expressed, Pramef12, while others are upregulated after fertilization (C), consistenly with the human PRAME family members on Figure 5. The results are supported by sequencing data by Xue, et al (2013) for human and mouse NLRP families (D, E) and mouse PRAME family (F). NLRP7 is upregulated after human 8-cell stage in both the microarray and sequencing dataset, in contrast to overall trend in the family (A, D). Mouse Nlrp12 is lowly expressed in both mouse datasets while the other genes are mostly higher expressed in the oocyte and 1-cell embryo (B, E). Mouse Pramef12 gene is maternally loaded in both TaqMan array and sequencing method in contrast to the rest of the genes in the family (C, F). Average log2(comparative expression) values for each stage were used for the human data obtained from Zhang et al., 2007 microarray expression dataset and average −ΔCt values were used for the mouse expression data produced in the current study. Undetected samples were attributed the −ΔCt value of −14.2. Human and mouse sequencing data from Xue, et al, 2013 shows average ln(RPKM+1) values for the same biological stage.
