Expression of the Tic22 genes in different tissues and at different developmental stages.

A. Quantitative RT-PCR analysis of total-RNA from whole seedlings grown for five days in the dark (5dD), or five and 14 days in the light (5dL and 14dL, respectively), as well as from three different tissues of mature plants (rosette leaves, siliques, and roots). RNA samples were representative of ∼10–30 seedlings (5dD, 5dL and 14dL), or 5–25 mature plants (rosettes, siliques and roots). Tic22 data were normalized relative to the control gene, ACTIN2 (At3g18780), and then expressed relative to the atTIC22-IV 5dL value. Data shown are means (± SE) derived from three biological replicates. B. Affymetrix GeneChip data were analysed and retrieved using the Genevestigator V3 analysis tool (https://www.genevestigator.com) [29], [60]. Presented data were prepared using the Development representation in scatter-plot format. Data from all high-quality ATH1 (22 k) arrays were analysed; this amounted to a total of 7392 samples. Values shown are means. The total number of samples used to derive each data point shown is indicated. Typical ranges of low, medium, and high expression for the array type are shown; medium is defined as the interquartile range (IQR). Stages of development are defined as follows, from left to right: germinating seed, seedling, young rosette, developed rosette, bolting, young flower, developed flower, flowers and siliques, mature siliques, and senescence. Data representations were exported from Genevestigator in portable document format, and then annotated using appropriate graphics software. The genes analyzed were as follows: atTIC22-IV (At4g33350; red); atTIC22-III (At3g23710; blue); atTIC110 (At1g06950; green); atTOC33 (At1g02280; orange); atTOC34 (At5g05000; purple).