Expression of genes associated to carbon fixation (Rubisco, rbcA) and synthesis of glycerol (glycerol 3-phosphate dehydrogenase, GPD).

RT-PCR from 1 µg RNA extracted from Symbiodinium type B17 (upper panel) or type A1 cultures (lower panel) exposed to control (left lanes) or osmotic stress conditions (right lanes) for 1 hr. The amplification products were separated in agarose gels photographed, scanned and quantified with the ImageJ program (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2011). Color was inverted in the scans for clearer visualization. Numbers represent relative intensity of bands normalized to transcript levels for GAPDH (glyceraldehyde 3-phosphate dehydrogenase, see methods).