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Expression of cytokines in porcine intestinal epithelial (PIE) cells after stimulation with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs).

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posted on 2013-03-27, 10:54 authored by Yohsuke Tomosada, Julio Villena, Kozue Murata, Eriko Chiba, Tomoyuki Shimazu, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Haruki Kitazawa

PIE cells were pre-treated with different bacterial strains for 48 h and stimulated with heat-stable ETEC PAMPs. The expression of MCP-1, IL-6, and IL-8 mRNAs (A) and proteins levels (B) were studied at 12 h post-stimulation. The results represent four independent experiments. Different letters indicate significant differences (P<0.05).

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